Fig. 2

MSC-EVs promoted the anabolic metabolism, proliferation and migration of NP cells. A Morphology of EVs observed by transmission electron microscopy (left panel) and diameter distribution evaluated by nanoparticle tracking analysis (right panel). Scale bar: 100 nm. B NP cells were treated with 10 μg/ml (EVs-10), 50 μg/ml (EVs-50), or 100 μg/ml (EVs-100) EVs for 24 h. Cell viability was evaluated by CCK-8 assay. C Expression of ACAN, COL2A1, MMP3, and MMP13 measured by western blot and the quantification of these protein levels (D). E Representative images of the wound healing assay in NP cells. The percentages of filled wounded area were quantified (right panel). Data were presented as mean ± SD of three independent replicates. ***P < 0.001