Fig. 3

Vasorin in EVs promoted the anabolic metabolism, proliferation and migration of NP cells. A NP cells were treated with 10, 50, or 100 μg/ml EVs for 24 h. The protein level of Vasorin was measured and quantified by western blot. B EVs were isolated from common MSCs (EVs-control), siRNA-VASN transfected MSCs (EVs-si-VASN) or scrambled siRNA transfected MSCs (EVs-si-scr). The protein levels of Vasorin, Alix and CD63 were measured in the MSCs and EVs fraction. The relative levels of vasorin in EVs (relative to CD63) was quantified (right panel). C NP cells were incubated with these EVs equivalently (50 μg/ml) for 24 h. The protein levels of Vasorin, ACAN, COL2A1, MMP3 and MMP13 were measured and quantified in NP cells (D). E Representative images showed the wound healing assay of NP cells treated with different kinds of EVs for 24 h. The percentages of filled wounded area were quantified (right panel). F The NP cell viability in different group was evaluated by CCK-8 assay. Data were presented as mean ± SD of three independent replicates. P > 0.05 (ns not significant), **P < 0.01, and ***P < 0.001