Fig. 6

Fabrication and characterization of FEC hydrogel. A Images of F127, F127 with 0.1% or 1% dECM and dECM in tilted bottle at 4, 25, 37 °C. B Variation of storage modulus G’, loss modulus G’’ and viscosity of hydrogels as the temperature changes. C EVs (50 μg/ml) were encapsulated into FEC hydrogel (EVs@FEC), and the G’ and G’’ were evaluated by a rheometer. D The protein level of released EVs in FEV hydrogel was measured by BCA protein assay, and compared with the degradation rate in PBS. E Live/Dead staining of NP cells cultured on the FEC hydrogel and common culture dish. F The cell viability of NP cells cultured in common dish or FEC was evaluated by CCK-8 assay. Data were presented as mean ± SD of three independent replicates. P > 0.05 (ns, not significant)