Fig. 1

Characterization of exosomes and their internalization into NRCMs. A Representative images of CON-EXO and HHP-EXO under transmission electron microscopy (inlets) and their size distribution by NanoSight NS300. B Western blots showing the significant expression of the exosome markers CD9, TSG101 and Alix in CON-EXO and HHP-EXO. C Exosomes were immunoprecipitated with Anti-FLAG affinity resin and probed with antibodies against the exosome markers CD9, TSG101 and Alix. D Representative images showing the internalization of CM-Dil-labeled HHP-EXO or CON-EXO (red) into FITC-phalloidin stained NRCMs (green) and DAPI counter-stained nuclei (blue). E Typical flow cytometry plots showing the internalization of CM-Dil-labeled HHP-EXO or CON-EXO into NRCMs after 24 h incubation. F Mean fluorescence intensity in E (n = 3). Data are presented as ‘Mean ± SEM’, **P < 0.01