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Fig. 5 | Journal of Nanobiotechnology

Fig. 5

From: Small extracellular vesicles of hypoxic endothelial cells regulate the therapeutic potential of adipose-derived mesenchymal stem cells via miR-486-5p/PTEN in a limb ischemia model

Fig. 5

miR-486-5p meditated the cell protective and proangiogenic effects of hsEVs. A Venn diagram of the potential miRNAs targeting PTEN. B Quantification of a series of miRs in nsEVs and hsEVs by qRT–PCR. C Expression of miR in ADSCs treated with nsEVs or hsEVs was measured by qRT–PCR. D The viability of ADSCs was monitored by CCK8 assay. E, F Apoptosis rates of ADSCs under ROS exposure were measured by FCM. G A scratch assay of endothelial cells was conducted in the presence of different CM from ADSCs for 48 h. H Representative phase-contrast images of the matrigel tube formation assay. I, J Relative expression of PTEN in ADSCs was analyzed using western blotting. K MiR-486-5p regulated PTEN by directly targeting the 3′-UTR. L A dual Luciferase reporter containing wild-type or mutant PTEN 3′UTR and scramble microRNA (mimic-NC) or miR-486-5p mimic was co-transfected into HEK293 cells. All data are representative of three independent experiments and are shown as the mean ± SEM. (n = 3; *P < 0.05; **P < 0.01).

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