Fig. 7

hsEVs enhanced the therapeutic effects of ADSCs in CLI. A Representative images of GC muscle stained for CD31 (red) and DAPI (blue). B Quantitative analysis of the capillary density in each group. C Laser Doppler perfusion image of ischemic hindlimb as indicated time after surgery. D Quantitation of perfusion recovery of the hindlimb. E Representative images of H&E staining and Masson’s staining of GC muscle sections in each group. F Collagen content (blue) via Masson’s trichrome staining of the GC muscle was calculated. G Tissue damage and motor function scores of the various treatment groups over time. Data are expressed as the mean ± SEM. (n = 5. *P < 0.05 versus PBS; ^#P < 0.05 versus ADSCs; ^$P < 0.05 versus hsEVs-ADSCs).