Fig. 5

A Fluorescence images showing reduction of intracellular ROS with ROS staining by DCFH-DA probe. B Effect of the PBNPs@OBG on mitochondrial membrane potential in NP cells by JC-1 staining. Scale bar = 50 µm. C–D Effect of the PBNPs@OBG on ROS levels after treated with H2O2 determined by flow cytometry on NPs. E–F Expressions of SOX9, collagen II, MMP3, and MMP13 were determined by WB E and qRT-PCR F of NPs in vitro (n = 3, *P < 0.05, **P < 0.01, ***P < 0.001)