Fig. 3

A. Expression of ULBPs on prostate cancer cells after Ferumoxytol treatment. Cancer cells were treated with ferumoxytol for 24 h and, expression of ULBPs was measured by flow cytometry. B. MHC class I and II expression on PC-3 prostate cancer cell were determined by flow cytometry C. HMGB1 expression of each treatment was determined by flow cytometry. Cancer cells were co-cultured with mouse primary NK cells with or without ferumoxytol at a 1:1 effector: target ratio, and HMGB1 expression was measured. D. Cell-surface expression of PD-L1 in response to ferumoxytol mediated ferroptosis, as determined by flow cytometry. E. NK cell tumor cell killing effect of each treatment (only NK cells, ferumoxytol + NK cells, and ferumoxytol + NK cells + aPD-L1) was determined by CFSE/7AAD assay. F. Interferon gamma secretion after each treatment (only NK cells, ferumoxytol + NK cells, and ferumoxytol + NK cells + aPD-L1). The data represent mean ± s.d. (n = 3) and statistical significance was analyzed by two-tailed Student’s t-tests. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001