Fig. 6

Remodeling of tumor immune environment in responses of NK cell and ferumoxytol mediated ferroptosis combined therapy. A. Experimental design of in vivo immune remodeling effect in syngeneic mice model. The 6–8-week-old male C57BL/6 mice were randomly divided into 4 groups, DPBS, mpNK, mpNK + Fer and mpNK + Fer-aPDL1 group. TrampC1 bearing C57BL/6 mice were injected NK cells intratumorally 4 times on days of 28, 32, 35 and 39. B. Flow cytometry analysis of the percentage of NK cells in the tumors, the amount of NK cells containing IFN-γ and the ratio of CD8 + T cell/ regulatory T cell in the tumors after indicated treatment. The data represent mean ± s.d. (n = 4) and statistical significance was analyzed by two-tailed Student’s t-tests. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001