Fig. 4

Isolation, identification, and modification of huc-MSCs. A Inverted phase contrast microscopy images of cells morphological observation of huc-MSCs. Scale bar: 100 µm. B Identification of huc-MSCs with CD45, CD73, CD105, and HLA-DR. C Sequencing results of fused gene of Lamp2b and HSTP1 constructed in plasmid vectors. Sequence of yellow shading was GNSTM, sequence of red shading was HSTP1. D Fluorescence microscope images of infection efficiency of empty vector lentivirus (negative control), Lamp2b gene overexpression lentivirus (Lamp2b +), and fused gene overexpression lentivirus (Lamp2b + HSTP1) in huc-MSCs. E RT-PCR results of Lamp2 mRNA expression in Blank control, Negative control, Lamp2b + , and Lamp2b + HSTP1 with the universal primer (forward 5’-AACCCCAATACAACTCACTCC-3’, reverse 5’-GCCATTAACCAAATACATGCTG-3’). F Agarose gel electrophoresis of RT-PCR products with the universal primer. G Agarose gel electrophoresis of RT-PCR products with the specific primer (forward 5’-AGGAAACTCCACCATGTGTGATG-3’, reverse 5’-GCTTCCATTATATGTCACAGTGCC-3’). H Western blot and relative protein expression qualification for the Lamp2 in huc-MSCs. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001