Nanoengineering facilitating the target mission: targeted extracellular vesicles delivery systems design

Song, Haoyue; Chen, Xiaohang; Hao, Yujia; Wang, Jia; Xie, Qingpeng; Wang, Xing

doi:10.1186/s12951-022-01638-9

Journal of Nanobiotechnology

Table 1 Surface-displayed proteins of EVs for targeted therapy

From: Nanoengineering facilitating the target mission: targeted extracellular vesicles delivery systems design

Protein on the surface of EV	Targeting peptide	Parent cell	Function	Effect	Measures of targeting efficiency	Refs.
Lamp-2b	RVG	Primary immature Dendritic cells	Alleviate Alzheimer’s disease	Central nervous system specific RVG binds specifically to acetylcholine receptor	Confocal laser imaging colocalization	[24]
	RGD	Leukemia cell line K562	Promoted therapeutic angiogenesis	Overexpression of αvβ3 integrin on the surface of blood vessels, which specifically binds to RGD peptide	Targeted metabolic labeling technique	[107]
	IMTP	BMSC	Enhanced vasculogenesis, and cardiac function	IMTP modified EVs can preferentially target ischemic myocardium	Near-Infrared Fluorescence Tracer	[108]
	CMP	CDCs	Modulating the cardiac remodeling	CMP modified EVs can preferentially target cardiomyocyte	Whole-organ fluorescence imaging	[109]
	RGE	Raw264.7	Inhibit glioma	Cross the BBB smoothly, accurately identify gliomas	Fluorescence imaging	[110]
	CAP	Dendritic cells	Alleviate OA progression	CAP peptide modified EVs can specifically target chondrocytes	Near-Infrared Fluorescence Tracer	[25]
	T7	HEK293T cells	Treatment of glioblastoma	T7 peptide has a higher efficiency of brain targeting than RVG peptide	Fluorescence imaging	[111]
	tLyP-1	HEK293T cell	Treatment for non-small cell lung cancer	tLyP-1 peptide can selectively target nerve NRP1 and NRP2	–	[112]
	E7	Dendritic cells	Alleviate OA progression	E7 peptide can target SF-MSC	Fluorescence imaging	[113]
	IL-3	HEK293T cell	Improve prognosis in CML patients	IL-3 can specifically bind to IL-3R overexpressed in CML	Fluorescence imaging	[114]
	Her2-binding affibody	HEK293T cell	Reverse the drug resistance in colorectal cancer	Her2-binding affibody binds to Her2 overexpressed on the surface of HCT-116^5FR cancer cells	Fluorescence imaging	[46]
PDGFR	GE11	HEK293T cell	Deliver let-7a to breast cancer tissues that highly express EGFR	The specific binding ability of GE11 peptide to cancer cells overexpressing EGFR is better than EGF	In vivo imaging system monitoring	[104]
CD63	myostatin propeptide	NIH3T3	Treatment for DMD	Propeptide can be anchored on the surface of EVs via CD63	Flow cytometric analysis	[115]
CD63	OVA antigen	293-F cells	Improving DNA vaccine immunogenicity	OVA antigen fused to CD63 plasmid to produce EVs of antigen carriers that target CD8⁺ T cells to enhance immune response	–	[116]

RVG, Rabies viral glycoprotein; RGD, L-Asparticacid, L-arginylglycyl; IMTP, Ischemic myocardium-targeting peptide; BMSC, Bone Marrow Stromal Cells; CDCs, Cardiosphere-derived cells; CMP, Cardiomyocyte specific peptide; Raw264.7, Leukemia cells in mouse macrophage; BBB, Blood–brain barrier; CAP, Chondrocyte-affinity peptide; OA, Osteoarthritis; HEK293, Human embryonic kidney 293; SF-MSC, Synovial fluid-derived mesenchymal stem cells; NRP1, Neuropilin1; NRP2, Neuropilin2; IL3, Interleukin 3; CML, Chronic myeloid leukemia; HCT-116^5FR, 5-FU-resistant derivative of the HCT-116 human colon cancer cell line; EGFR, Epidermal growth factor receptor; EGF, Epidermal growth factor; DMD, Duchenne muscular dystrophy; TRAIL, TNF-related apoptosis-inducing ligand

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ISSN: 1477-3155

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