Fig. 2

Characterization of PMVs@PLGA complexes in vitro. A Transmission electron microscopy images of PMVs, PLGA and PMVs@PLGA complexes (scale bars indicate 500 nm). B NTA analysis of PMVs, PLGA and PMVs@PLGA complexes. C Agarose gel electrophoresis to identify complexes with different N/M ratios (N: nanoparticles; M: miRNAs). D In vitro cumulative release profiles of the miRNA from the PLGA-miRNA complexes in PBS at pH 7.2. E Protein Coomassie blue staining images of PMVs, PLGA and PMVs@PLGA complexes. F Detection of key platelet membrane markers on PMVs, PLGA and PMVs@PLGA complexes. G Validation of PMVs, PLGA and PMVs@PLGA complexes against H9C2 cytotoxicity by CCK-8 assays. H Fluorescent micrographs of thick smears of PMVs@PLGA (PMVs labelled using PKH67, PLGA labelled using rhodamine) (scale bars indicate 10 μm). I Flow cytometry assay for particle uptake efficiency of PMVs, PLGA and PMVs@PLGA complexes into H9C2 cells (PMVs using Dil labelling, PLGA using FITC labelling). ns no significance