Fig. 5

MiRNA regulation of Nrf2 in MIRI (1). A qPCR assay of Nrf2 expression in tissues. B Fluorescence micrographs and quantitative analysis of IF detection of Nrf2 expression in tissues (scale bars indicate 100 μm). C Fluorescence micrographs and quantitative analysis of IF assays for Nrf2 expression in PMVs@PLGA-treated myocardium (scale bars indicate 100 μm). D Prediction results for Nrf2 mRNA-binding miRNA from two databases, TargetScan and miRDB. E qPCR assay of 14 miRNAs expressed in tissues. ns no significance; *P < 0.05; **P < 0.01; *** P < 0.001; **** P < 0.0001