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Table 1 Performance of luminescence and fluorescence-based DNAzyme-assisted aptasensors for cancer detection

From: The recent advancements in the early detection of cancer biomarkers by DNAzyme-assisted aptasensors

Detection Method

Biomarkers/cells

Strategy

Sample

Aptamer Sequence

(5′ → 3′)

NM/NPs

LOD

LR

Refs.

Luminescence

CEA

A quenching probe containing DNAzyme/ AuNRs-cDNA

Serum

NH2-(CH2)6-TTTTATACCAGCT

TATTCAATT

AuNRs

0.036 pg mL− 1

0.1 pg mL− 1 − 0.5 ng mL− 1

[41]

A dual amplification by CHA and HCR

Serum

TACCAGCTTATTCAATT

-

8 fg mL− 1

8 fg mL− 1 − 0.50 pg mL− 1

[42]

a dual CEA/hemin conjugated aptamers/HRP-like G4-DNAzyme

Serum/Blood

CH-1: AAAGGTAGGGCGGGT

TGGGTAAATAAAAAAGGGGGT

GAAGGGATACCC

CH-2: TACCAGCTTATTCAATTA

AAAATAAAGGGTAGG

GCGGGTTGGGTAAAT

-

0.58 ng mL− 1

0-200 ng mL− 1

[43]

Pb2+-assisted DNAzyme/GQDs-IL-NF nanocomposite

Plasma

CATCTCTTCTCCGAGCCGGTC

GAAATAGTGAGTATACCAGCTTA

TTCAATTAAGAGATG

GQDs

0.34 fg mL− 1

0.5 fg mL− 1 to 0.5 ng mL− 1

[44]

graphitic carbon nitride (g-C3N4) nanosheets and DNAzyme

Serum

GAATAAGCTTCCACCA

TCCATACCAGCTTATTCTATT

g-C3N4 nanosheets

63.0 pg mL− 1

0.1–150 ng mL− 1

[49]

PDGF

Exo III-CRA based label-free CL aptasensors

Serum

CTCAGGCTACGGCACGTAGAG

CATCACCATGATCCTGAG

-

6.8 × 10− 13 M

-

[55]

VEGF

T7 exonuclease, CdS:Eu NCs, andG4/hemin DNAzyme

Blood

GGCCCGTCCGTATGGTGG

GTGTGCTGGCC

CdS:Eu nanocrystals

0.2 pM

1 pM to 20 nM

[62]

PSA

MOF/Au/G4 DNAzyme

Serum

(CH2)6-TTTTTAATTAAA

GCTCGCCATCAAATAGCTTT

AuNPs

0.058 ng mL− 1

0.5 to 500 ng mL− 1

[64]

miR-205

G4 DNAzyme/

ODI-CL/Amplex Red/H2O2

Serum/Blood

UCCUUCAUUCCA

CCGGAGUCUG

-

0.13 nM

0.4–62.5 nM

[70]

miR-155 miR-21 p53/BRCA1 genes

CRET-based method/ UiO-66 MOF-NPs/luminol/H2O2

Serum

miR-155: UUAAUGCUAAUCGU

GAUAGGGGU

miR-21: UAGCUUAUCAGAC

UGAUGUUGA

p53: TCATCACACTGGAAGACTC

BRCA1:AAAGTGTTTTTCATAAACCCATTATCCAGGACTGTTTATAGCTGTTGGAAG

MOF-NPs

-

-

[71]

BRCA1

HRP-mimic DNAzyme

Buffer

AGGGCGGGTGGGTGTTTAAGTT

GGAGAATTGTACTT

AAACACCTTCTTCTTGGGT

-

1 × 10− 13 M

-

[75]

Exosomal biomarker

MPA-CdS:Eu NCs/DNAzyme

Plasma

CD63 aptamer: CACC

CCACCTCGCTCCCGTGA CACTAA

TGCTATTTTTTTTTT-(CH2)7-NH2

CdS:Eu nanocrystals

7.41 × 104 particle mL− 1

3.4 × 105 to 1.7 × 108 particle mL− 1

[78]

Fluorescence

BRCA1

Mg2+-dependent DNAzyme

Buffer

TTGCTCCCTGTTGCTGAAAC

CATACAGCTTCATAAATA

ATTTTGCTT

-

1 × 10− 14 M

-

[81]

PDGF

PET between DNA-Ag NCs and G4/hemin

Serum

CAGGCTACGGCACGTAGAGCATCACCATGATCCTGTTTT

Ag NCs

1 × 1013 M

5 × 1013 to 1 × 108 M

[82]