From: The recent advancements in the early detection of cancer biomarkers by DNAzyme-assisted aptasensors
Detection Method | Biomarkers/cells | Strategy | Sample | Aptamer Sequence (5′ → 3′) | NM/NPs | LOD | LR | Refs. |
---|---|---|---|---|---|---|---|---|
Luminescence | CEA | A quenching probe containing DNAzyme/ AuNRs-cDNA | Serum | NH2-(CH2)6-TTTTATACCAGCT TATTCAATT | AuNRs | 0.036 pg mL− 1 | 0.1 pg mL− 1 − 0.5 ng mL− 1 | [41] |
A dual amplification by CHA and HCR | Serum | TACCAGCTTATTCAATT | - | 8 fg mL− 1 | 8 fg mL− 1 − 0.50 pg mL− 1 | [42] | ||
a dual CEA/hemin conjugated aptamers/HRP-like G4-DNAzyme | Serum/Blood | CH-1: AAAGGTAGGGCGGGT TGGGTAAATAAAAAAGGGGGT GAAGGGATACCC CH-2: TACCAGCTTATTCAATTA AAAATAAAGGGTAGG GCGGGTTGGGTAAAT | - | 0.58 ng mL− 1 | 0-200 ng mL− 1 | [43] | ||
Pb2+-assisted DNAzyme/GQDs-IL-NF nanocomposite | Plasma | CATCTCTTCTCCGAGCCGGTC GAAATAGTGAGTATACCAGCTTA TTCAATTAAGAGATG | GQDs | 0.34 fg mL− 1 | 0.5 fg mL− 1 to 0.5 ng mL− 1 | [44] | ||
graphitic carbon nitride (g-C3N4) nanosheets and DNAzyme | Serum | GAATAAGCTTCCACCA TCCATACCAGCTTATTCTATT | g-C3N4 nanosheets | 63.0 pg mL− 1 | 0.1–150 ng mL− 1 | [49] | ||
PDGF | Exo III-CRA based label-free CL aptasensors | Serum | CTCAGGCTACGGCACGTAGAG CATCACCATGATCCTGAG | - | 6.8 × 10− 13 M | - | [55] | |
VEGF | T7 exonuclease, CdS:Eu NCs, andG4/hemin DNAzyme | Blood | GGCCCGTCCGTATGGTGG GTGTGCTGGCC | CdS:Eu nanocrystals | 0.2 pM | 1 pM to 20 nM | [62] | |
PSA | MOF/Au/G4 DNAzyme | Serum | (CH2)6-TTTTTAATTAAA GCTCGCCATCAAATAGCTTT | AuNPs | 0.058 ng mL− 1 | 0.5 to 500 ng mL− 1 | [64] | |
miR-205 | G4 DNAzyme/ ODI-CL/Amplex Red/H2O2 | Serum/Blood | UCCUUCAUUCCA CCGGAGUCUG | - | 0.13 nM | 0.4–62.5 nM | [70] | |
miR-155 miR-21 p53/BRCA1 genes | CRET-based method/ UiO-66 MOF-NPs/luminol/H2O2 | Serum | miR-155: UUAAUGCUAAUCGU GAUAGGGGU miR-21: UAGCUUAUCAGAC UGAUGUUGA p53: TCATCACACTGGAAGACTC BRCA1:AAAGTGTTTTTCATAAACCCATTATCCAGGACTGTTTATAGCTGTTGGAAG | MOF-NPs | - | - | [71] | |
BRCA1 | HRP-mimic DNAzyme | Buffer | AGGGCGGGTGGGTGTTTAAGTT GGAGAATTGTACTT AAACACCTTCTTCTTGGGT | - | 1 × 10− 13 M | - | [75] | |
Exosomal biomarker | MPA-CdS:Eu NCs/DNAzyme | Plasma | CD63 aptamer: CACC CCACCTCGCTCCCGTGA CACTAA TGCTATTTTTTTTTT-(CH2)7-NH2 | CdS:Eu nanocrystals | 7.41 × 104 particle mL− 1 | 3.4 × 105 to 1.7 × 108 particle mL− 1 | [78] | |
Fluorescence | BRCA1 | Mg2+-dependent DNAzyme | Buffer | TTGCTCCCTGTTGCTGAAAC CATACAGCTTCATAAATA ATTTTGCTT | - | 1 × 10− 14 M | - | [81] |
PDGF | PET between DNA-Ag NCs and G4/hemin | Serum | CAGGCTACGGCACGTAGAGCATCACCATGATCCTGTTTT | Ag NCs | 1 × 1013 M | 5 × 1013 to 1 × 108 M | [82] |