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Fig. 1 | Journal of Nanobiotechnology

Fig. 1

From: Targeted OUM1/PTPRZ1 silencing and synergetic CDT/enhanced chemical therapy toward uveal melanoma based on a dual-modal imaging-guided manganese metal–organic framework nanoparticles

Fig. 1

OUM1 overexpressed in melanoma tissues and UM cells and OUM1 knockdown inhibited cell proliferation and invasion. a Hierarchical clustering analysis of 223 lncRNAs that were differentially expressed between melanoma samples and nontumor samples. b The lncRNA OUM1 was significantly overexpressed in melanoma tissues compared with normal tissues. c OUM1 showed higher expression in the melanoma cell lines OCM1, OCM1a and OM431 than in RPE cells (negative control), as measured by qRT-PCR. d OUM1 expression was measured by RT-PCR in different UM and normal cells. Triplicate assays were performed and the relative level of OUM1 was normalized to GAPDH (*P < 0.05). e OUM1 was knocked down by one shRNA. EGFP was applied to track OUM1 shRNA expression. f, g OUM1 was downregulated in UM cells, as shown by qRT-PCR and RT-PCR. h Images of the Transwell assays. i Migrated cells that were fixed with 5% paraformaldehyde, stained with 0.1% crystal violet and washed with 33% acetic acid were quantified. The absorbance of the collected liquid was measured at 570 nm with a microplate reader. j Images of colony formation assays. k Colonies were fixed, stained, washed and measured with a microplate reader at 570 nm. l Images of colonies in soft agar. Few small colonies were observed in the OUM1 knockdown group. m Nu/Nu nude mice were injected with OCM1a or shOUM1-OCM1a cells. Tumor volume was measured twice a week, every 3 or 4 days. The xenografts grew slowly in mice injected with shOUM1-OCM1a cells compared to those injected with control cells (n = 6 mice per group). n After 31 days, the mice were sacrificed, and the tumors were removed and analyzed. The tumors from mice injected with shOUM1-OCM1a cells weighted less. o Ki-67 expression was evaluated by IHC staining in mouse tumor tissues. Scale bar: 20 μm. Mock: empty pGIPZ vector; *P < 0.05 compared with the control

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