Fig. 2

OUM1 contributes to tumor progression through its downstream target PTPRZ1. a, b The mRNA expression of PTPRZ1 was upregulated in untreated UM cells but downregulated after OUM1 knockdown, as detected using qRT-PCR and native PAGE in OCM1a, OM431, shOUM1-OCM1a and shOUM1-OM431 cells. c The protein expression of PTPRZ1 decreased, as determined by western blot. d, e PTPRZ1 expression was higher in a panel of UM cell lines (OCM1, OCM1a, OM431, VUP, 92–1 and C918) than in control RPE cells as measured by qRT-PCR and RT-PCR. f, g PTPRZ1 expression was higher in a panel of UM cell lines than in normal RPE cells as shown by western blot. h In melanoma tissues, PTPRZ1 expression increased significantly, as detected by qRT-PCR. *P < 0.05 compared with the control. i, j Cell proliferation was significantly decreased in OCM1a and OM431 cells after PTPRZ1 silencing. k Images of metastatic PTPRZ1-silenced tumor cells. The migration assay was conducted 48 h after transfection with siPTPRZ1 or control siRNA. l, m Quantitative analysis of metastatic tumor cells. The migratory ability of siPTPRZ1-treated OCM1a and OM431 tumor cells was significantly decreased. n IHC staining confirmed that PTPRZ1 protein expression was decreased in mouse tumors formed by shOUM1-OCM1a cells. o PTPRZ1 expression was decreased in mouse tumors formed by shOUM1-OCM1a cells, as shown by qRT-PCR. *P < 0.05 compared with the control