Fig. 3

Targeting ability and functional regulation of PLP-RvD1 to BMDMs in vitro. A The binding ability of DiD-labeled LP-RvD1, PLP-RvD1 and anti-P-selectin blocked PLP-RvD1 to inflamed BMDMs was detected by fluorescent microscope and C statistically analyzed by ImageJ (n = 3 per group). Red, DiD (on liposome); Green, Cell membrane. Scalar bar, 10 µm. B The efferocytosis ability of BMDMs to apoptotic cardiomyocytes after PBS, LP-RvD1 or PLP-RvD1 treatment was detected by fluorescence microscopy images and D statistically analyzed by ImageJ (n = 3 per group). Green, Calcein AM-labeled BMDMs; Red, IVISense 680-labeled apoptotic cardiomyocytes. Scalar bar, 10 µm. E Quantification of capillary formation and cell migration in HUVECs after co-culture with PBS, LP-RvD1 or PLP-RvD1-treated Inflammed BMDMs (n = 3 per group). F Determination of related SPMs molecules (RvD1, RvD2, RvE1, LXA4) in culture medium of efferocytosis assay by ELISA (n = 3 per group). Results are presented as mean ± SD. ^NSP > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001