Fig. 3

AGuIX nanoparticles increase DNA damage by inhibiting homologous recombinant repair proteins in MDA-MB-231 breast cancer cells. A Expression levels of the MRN complex in MDA-MB-231 cells via western blot analysis at the indicated postirradiation times. B Expression levels of ATM, p-ATM and p-Chk2 in MDA-MB-231 cells via western blot analysis at the indicated postirradiation times. C Expression levels of p53, p-p53 in MDA-MB-231 cells via western blot analysis at the indicated postirradiation times. D Homologous recombinant repair protein (BRCA1) and non-homologous end-linked repair protein (KU70/80) expression levels in MDA-MB-231 cells via western blot analysis at the indicated postirradiation times. All data are represented as means ± standard errors of the means, n ≥ 3 independent replicates and P-values were calculated using two-tailed t-tests. A single asterisk indicates P < 0.05, two asterisks indicate P < 0.01, and three asterisks indicate P < 0.001