Fig. 5

AGuIX nanoparticles combined with radiation induce ferroptosis in breast cancer cells. A Ultrastructure of MDA-MB-231 breast cancer cells under an electron transmission microscope 24 h after irradiation. B Representative images of the colony formation of the MDA-MB-231 cells after different treatments. C Representative images of the colony formation of the MDA-MB-468 cells after different treatments. D Survival curves of the MDA-MB-231 cells after different treatments. E Survival curves of the MDA-MB-468 cells after different treatments. F Lipid peroxidation levels of the MDA-MB-231 cells cells 24 h after irradiation. G Lipid peroxidation levels of the the MDA-MB-468 cells 24 h after irradiation. H The expression of the 4-hydroxynonenal protein in the tumor tissues was analyzed by immunohistochemistry. All data are represented as means ± standard errors of the means, n ≥ 3 independent replicates and P-values were calculated using two-tailed t-tests. A single asterisk indicates P < 0.05, two asterisks indicate P < 0.01, and three asterisks indicate P < 0.001