Fig. 6

AGuIX nanoparticles induce more ferroptosis in breast cancer cells by inhibiting the cysteine transport system. A FTH1/TFR1 protein levels via western blot analysis at the indicated postirradiation times. B ACSL4 protein levels via western blot analysis at the indicated postirradiation times. C NRF2 protein levels via western blot analysis at the indicated postirradiation times. D Ratio of reduced glutathione to oxidized glutathione in MDA-MB-231 cells with different treatments. E Ratio of reduced glutathione to oxidized glutathione in MDA-MB-468 cells with different treatments. F GPX4 protein levels via western blot analysis at the indicated postirradiation times. G GPX4 activity in MDA-MB-231 cells with different treatments. H GPX4 activity in MDA-MB-468 cells with different treatments. All data are represented as means ± standard errors of the means, n ≥ 3 independent replicates and P-values were calculated using two-tailed t-tests. A single asterisk indicates P < 0.05, two asterisks indicate P < 0.01, and three asterisks indicate P < 0.001