Fig. 7

NRF2, SLC7A11, and GPX4 were knocked down or overexpression in MDA-MB-231 cells, respectively, and their effects on the radiation sensitization of AGuIX nanoparticles were verified. A, E, I The knockdown efficiency of siRNAs was examined by polymerase chain reaction assays and immunoblotting assays. B, F, J, N Cell proliferation of different treatment groups after ionizing radiation. C, G, K, O Representative images of lipid peroxidation levels of MDA-MB-231 cells after different treatments. D, H, L, P lipid peroxidation levels of MDA-MB-231 cells after different treatments. M The protein expressions of NRF2, SLC7A11 and GPX4 in MDA-MB-231 cells after overexpression of NRF2. All data are represented as means ± standard errors of the means, n ≥ 3 independent replicates and P-values were calculated using two-tailed t-tests. A single asterisk indicates P < 0.05, two asterisks indicate P < 0.01, and three asterisks indicate P < 0.001