Fig. 4

IGF1R is a direct target of miR-140-5p. a 191 potential target genes overlapped in the predictions by TargetScan and miRDB. b Relative expression of IGF1R assessed by qPCR after treatment with miR-140-5p-exo. c Diagram of IGF1R 3’UTR reporter constructs. d Luciferase reporter assays in hMSCs transfected with wt or mt IGF1R 3’UTR and exosomes, as indicated. The relative expression of IGF1R after treatment with miR-140-5p mimic or inhibitor was assessed by qPCR e and Western blot f. All experiments were repeated three times. GAPDH served as an internal control. *P < 0.05. All data are presented as the mean ± SD. wt, wild-type. mt, mutant type