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Fig. 4 | Journal of Nanobiotechnology

Fig. 4

From: Targeting Myd88 using peptide-loaded mesenchymal stem cell membrane-derived synthetic vesicles to treat systemic inflammation

Fig. 4

SyEV are taken up by macrophages through active endocytosis. a DiO-labelled SyEV (green; 1 × 109) were incubated with RAW 264.7 cells for 6 h. Cell membranes and nuclei were stained by Cellmask Deep Red (red) and DAPI (blue), respectively. Scale bars, 20 μm. b The uptake of SyEV by cells was compared with NV by flow cytometry, and the results are shown as the percentage of DiO-positive cells (n = 3). c The macrophages were preincubated with Dynasore for 1 h at 37 °C, followed by treatment with DiO-labelled SyEV for 6 h at 37 °C. In parallel, SyEV were added to the cells for 6 h at 4 °C. The uptake of the labelled SyEV by cells was analyzed by flow cytometry (n = 3). Data are presented as the mean ± SEM. ***P < 0.001; ns, not significant, by one-way ANOVA with Tukey’s post test

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