Fig. 1

Treatment of tumor and normal cells with pDMP-T5 and FeNPs. Cells (BGC823, GES-1, HepG2, HL7702, KG-1a, WEHI-3) were transfected by DMP vectors overnight, then incubated with or without 50 µg/mL FeNPs for 72 h. a Schematic diagram of FAST eradicating tumor. b Cells stained by AO&EB. c Cell viability. The images and viability of cells incubated with FeNPs for 24 and 48 h are shown in the Additional file 1: Figs. S1–6, in which the data of more treatments as controls are shown. Cell viability was detected by the CCK8 assay. d and e Iron content (d) and ROS levels (e) of cells incubated with 50 µg/mL FeNPs for 48 h. All values are mean ± s.d. (n = 3 wells). All statistical significance is labeled. pDMP-NT, a plasmid coding an artificial microRNA targeting no transcripts (NT); pDMP-T5, a plasmid coding artificial microRNAs targeting five target (T) genes including FSP1, FTH1, GPX4, SLC7A11, and NRF2.