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Fig. 3 | Journal of Nanobiotechnology

Fig. 3

From: Ferroptosis assassinates tumor

Fig. 3

FAST-induced cancer cell death by ferroptosis. a Lipid ROS imaging. Lipid ROS production was detected by C11-BODIPY and imaged by fluorescence microscope. Red, reduced dye; green, oxidized dye. Only the representative images of HepG2, KG-1a, and HL7702 that were incubated with 50 µg/mL FeNPs for 72 h post pDMP-NT/T7 transfection are shown here. More detailed pictures (including 5 cancer cells and 4 normal cells) and quantified results are shown in Additional file 1: Figs. S26‒30. b and c The quantified data of lipid peroxidation in HepG2 at 24, 48, and 72 h. The lipid peroxidation in cells were determined by quantitating the fluorescence intensities analyzed by ImageJ software and calculating the ratio of intensity in 590 to 510 channels. Fer-1, Ferrostain-1 (1 µM); DFO, deferoxamine (100 µM); NAC, N-acetylcysteine (1 mM); DFN (mixture of 1 µM Fer1, 100 µM DFO, and 1 mM NAC); BA1, Bafilomycin A1 (1 nM); Nec1s, Necrostatin-1s (10 µM); ZVAD, ZVAD-FMK (50 µM). d Cell viability of HepG2, HL7702 and KG-1a when co-incubated with FeNPs and cell death inhibitors for 72 h post plasmid transfection overnight. e Quantitative clone formation assays of the treated HepG2 when exposed to cell death inhibitors. Each treatment was conducted in triplicates. The images of cell clones are shown in Additional file 1: Fig. S31. f Schematics of animal treatment (WEHI-3 xenograft mice). s.c., subcutaneous injection; i.v., intravenous injection. g Tumor growth curve (n = 10 mice). h Kaplan-Meier survival curve (n = 10 mice). The statistical significance was analyzed by the log-rank test

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