Fig. 1

Localization of labeled phage-displayed peptides on the human lens capsule having XFS materials. M13 phages with XFS material-targeting peptides displayed on coat protein pIII and wild-type M13 phages were labeled with Cy5 fluorescent dye and incubated with human lens capsule containing XFS materials. Phages with displayed p-LPS (A) and p-IPL (C) peptides on their surface and labeled with Cy5 dye were selectively bound to the XFS materials on the lens capsule. Wild-type phages labeled with Cy5 dye (control) did not show any specific interaction with XFS materials (E). The presence of XFS materials on the lens capsule was confirmed in the bright field mode of the microscope (B, D, and F). Arrows show the exfoliative zones on the human lens capsule. (Scale bars = 100 µm)