Fig. 8

Cell viability (live/dead) assay of hTM cells incubated with free peptide and MP-peptide constructs. Calcein AM (CAM)/ EthD-1 system: Calcein AM detects cellular esterase activity of live cells, EthD-1 stains nuclei of dead cells. The green cells are live (CAM) and red cells are dead cells (EthD-1). (A, F) Control cells treated with water (without MPs). (B, G) hTM cells incubated with 50 µg MP-p-IPL for 24 h. (C, H) hTM cells incubated with 100 µg MP-p-IPL for 24 h. (D, I) hTM cells incubated with 50 µg MP-p-LPS for 24 h. (E, J) hTM cells incubated with 100 µg MP-p-IPL for 24 h. (K, L) hTM cells treated with 1 mM free p-IPL peptide solution (without MPs). (M, N) hTM cells treated with 1 mM free p-LPS peptide solution (without MPs). (O) All cells dead (methanol treated). Each treatment included three biological and three technical replicates. (Scales bars = 50 µm)