Fig. 2
From: Cytoplasmic delivery of siRNA using human-derived membrane penetration-enhancing peptide
Effect of endocytosis inhibitors on the intracellular delivery of the complex of RBDmut-S19-TAT protein with Cy3-labelled siRNA. a Confocal images of HeLa cells treated with the complex of 400 nM RBDmut-S19-TAT and 100 nM Cy3-labelled siRNA (Red) in the absence (Untreated) or the presence of 80 μM dynasore, 1 μM cytochalasin D, 100 nM wortmannin, 1 mM MβCD or 50 μM chlorpromazine for 1 h and observed on live cells. Nuclei were stained with Hoechst 33,342 (blue). Scale bars, 20 μm. b ROI was taken for each cell and the average fluorescence intensity of Cy3 was quantified. N = 52, 38, 38, 24, 41 or 34; means ± SD; **p < 0.01