Fig. 3

Gene knockdown with the RBD-S19-TAT protein/siRNA complex. a HeLa cells or b LNCaP cells were treated with RBD-S19-TAT loaded with siRNA against the AR gene (grey) or the CSK gene (white). After treatment, the total RNA was extracted and subjected to cDNA synthesis, and the relative expression of the CSK gene a or the AR gene b was determined by RT–qPCR using the comparative CT method based on the expression level of the housekeeping gene RPL37A. The relative expression levels of CSK a and AR b mRNA were normalized by the mRNA expression using lipofection of the negative-control siRNA against AR a or CSK b, respectively. N = 3; means ± SD; *p < 0.05, **p < 0.01