Fig. 4
From: Cytoplasmic delivery of siRNA using human-derived membrane penetration-enhancing peptide
Schematic representation of the cytoplasmic delivery of siRNA in this study. By using a RBD or b Ago2 as the carrier protein fused with the membrane-penetrating peptides S19 and TAT, siRNA/protein complexes can be incorporated into cells by macropinocytosis (Step 1) and escape from endosomes (Step 2). Although a siRNA must dissociate from RBD-S19-TAT (Step 2') and reassociate with Ago2 (Step 2") to form RISC (Step 3), b the TAT-S19-Ago2/siRNA complex is expected to act immediately as RISC (Step 3)