Fig. 2

Stability of LysoTracker Red probe under different imaging conditions. A Cell viability after incubation with LysoTracker Red probe for 1 h, 4 h and 24 h assessed via membrane rupture assay (lactate dehydrogenase; LDH) and presented as fold change over untreated cells (control). Data shows the mean of three replicates. Cells exposed to 0.2% Triton X-100 (v/v) served as positive control for LDH assay. B Schematic representation of staggered experimental set-up (cells were stained for 1 h with LysoTracker Red probe, the supernatant containing LysoTracker Red was removed and cells were further incubated and imaged at 1 h, 4 h and 24 h as hyperstacks) and continuous set-up (cells were stained for 1 h with LysoTracker Red probe and imaged continuously for 24 h as hyperstacks). C Comparison of raw integrated densities of LysoTracker Red probe versus time for continuous and staggered imaging. Each time-point (hour) shows the raw integrated density measured for each cell individually. The whiskers are the standard deviations. **** (P < 0.05) is the significant difference analyzed by One-way ANOVA