Fig. 3
From: Pitfalls in methods to study colocalization of nanoparticles in mouse macrophage lysosomes
Effect of spatial resolution, optical slice thickness, pixel saturation and bit depth on the Pearson’s correlation coefficients (PCC, white boxes) and Manders’ M1 coefficient (gray boxes). The data correspond to the experimental set-up where cells were exposed to 59 nm SiO2-BDP FL NP for 13 h, fixed and stained with Alexa Fluor 647-conjugated LAMP-2 antibody. The error bars represent the standard deviations. All the images were analysed using ImageJ with the JACoP plugin comparing the correlation coefficients after each specific simulation