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Table 1 Main LysoTracker probes characteristics and experimental parameters

From: Pitfalls in methods to study colocalization of nanoparticles in mouse macrophage lysosomes

LysoTracker*

Excitation (nm)1

Emission (nm)1

Recommended concentration (nM)

Incubation time (37 °C)

Refs.

Green (DND-26)

504

511

50

15–30 min

[47,48,49]

Blue (DND-22)

373

422

50–100

30 min–1.5 h

[50,51,52]

Red (DND-99)

577

590

10–50

15 min–4 h

[19, 30, 53]

  1. 1Wavelength. The information in this table refers to LysoTracker probes purchased from ThermoFisher Scientific.
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Journal of Nanobiotechnology

ISSN: 1477-3155

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