Fig. 5

Cellular biocompatibility of DPCs co-cultured with IPN GC hydrogels and IGMs. (a) DPCs for three groups (green represents live cells, and red indicates dead cells). (b) Statistical diagram of cell viability % for DPCs (NS, no significance). (c) Statistical diagram of cell proliferation activity (*p < 0.05; **p < 0.01 IPN GC hydrogels + 20% PRP relative to the control group; #p < 0.05; ##p < 0.01 IPN GC hydrogels + 20% PRP relative to IPN GC hydrogels; ++p < 0.01, day 7 relative to day 1 and day 4). (d) DPCs seeded on IGMs and IGMs + 20% PRP for 1, 4, and 7 days detected by live/dead staining. Statistical diagram of cell viability (e) and cell proliferation activity (f) of DPCs in two groups (*p < 0.05 related to IGM; **p < 0.01 related to IGMs; #p < 0.05, day 7 relative to day 1 and day 4). (g) 3D confocal fluorescence microscopy images showing the mutual adhesion of IGMs (red represents DPCs). Statistical significance was analyzed using a one-way ANOVA for three groups and unpaired independent-samples t-test for two groups; n = 3 for each group