Fig. 3

Anticancer mechanism of PSSP@ART-ISMN. A–C Intracellular ROS, NO, and RNS levels were detected by DCFH-DA, DAF-FM-DA, and O52D bio-probes, respectively, scale bar = 50 µm. D–F Flow cytometry analysis of intracellular ROS, NO, and RNS generation. G Western blot analysis of proteins related to mitochondria-dependent apoptosis, DNA damage, and cell cycle arrest. H Cell cycle study and quantitative analysis of SKOV3 cells after various treatments