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Fig. 2 | Journal of Nanobiotechnology

Fig. 2

From: A split influenza vaccine formulated with a combination adjuvant composed of alpha-d-glucan nanoparticles and a STING agonist elicits cross-protective immunity in pigs

Fig. 2

Representative gating strategy for the analysis of lymphocytes and myeloid cells in TBLN MNCs. Five-week-old SPF pigs were administered twice ID/IM with NanoS100-SwIAV, Nano11-SwIAV or control Nano11S100 and challenged at post-prime vaccination day 35 with SwIAV H1N1-OH7. PBMCs, TBLN MNCs, and BAL cells were isolated at DPC6 and were restimulated with SwIAV H1N1-OH7 in vitro. The cells were labeled and analyzed by flow cytometry for the frequencies of different types of myeloid and lymphocyte subsets. A CXCL10+ myeloid cells; B IFNγ+ early/late effector CTLs and central/effector memory T-helper/memory T-helper cells; C IL-17A+ early/late effector CTLs and central/effector memory T-helper/memory T-helper cells

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