Fig. 3

NanoS100-SwIAV modulated activated myeloid immune cell frequencies in vaccinated pigs. Five-week-old SPF pigs were administered twice ID/IM with NanoS100-SwIAV, Nano11-SwIAV or control NanoS100 and challenged at post-prime vaccination day 35 with SwIAV H1N1-OH7. TBLN MNCs, BAL cells, and PBMCs were isolated at DPC6 and were restimulated with SwIAV H1N1-OH7 in vitro. The cells were labeled and analyzed by flow cytometry for the frequencies of activated myeloid cell subsets: A Myeloid cells-ID [CD3⁻CD172⁺CXCL10⁺]; B Plasmacytoid dendritic cells-ID [CD3⁻CD172⁺CXCL10⁺CD4⁺CD80/86⁺]; C Myeloid cells-ID [CD3⁻CD172⁺CXCL10⁺CD4⁻CD80/86⁺] in TBLN MNCs; D Plasmacytoid dendritic cells-ID [CD3⁻CD172⁺CXCL10⁺CD4⁺CD80/86⁺]; E Myeloid cells-IM [CD3⁻CD172⁺CXCL10⁺CD4⁻CD80/86⁺]; F Plasmacytoid dendritic cells-IM [CD3⁻CD172⁺CXCL10⁺CD4⁺CD80/86⁺] in BAL cells; G Myeloid cells-ID [CD3⁻CD172⁺CXCL10⁺]; H Plasmacytoid dendritic cells-ID [CD3⁻CD172⁺CXCL10⁺CD4⁺CD80/86⁻]; and I Myeloid cells-IM [CD3⁻CD172⁺CXCL10⁺] in PBMCs. Data represent the mean value of 7–8 pigs ± SEM. Statistical analysis was performed by one-way ANOVA followed by Tukey’s post-test. For head-to-head comparison between two groups, Unpaired t-test was used. Asterisk/Pound represents significant difference between indicated groups (*/♯ p < 0.05, **/♯♯ p< 0.01, and ***/♯♯♯ p < 0.001)