Fig. 3
A In vitro cell viability of 4T1 and HC11 cells treated with various concentration of FEP or FEPT in the dark. B Cell viability of 4T1 cells treated with FEP or FEPT with irradiation (808 nm, 1.0 W/cm2). C Live/dead staining of FEP or FEPT treated 4T1 cells with 808 nm laser irradiation (1.0 W/cm2) for 10 min. The live cells were stained by Calcein-AM (green), whereas dead cells were stained by PI (red). Scale bar: 100 μm. D Cleaved caspase-3 staining of FEP or FEPT treated 4T1 cells with 808 nm laser irradiation (1.0 W/cm2) for 10 min by CLSM. The primary antibody is cleaved caspase-3 polyclonal antibody, while the second antibody is Goat Anti-Rabbit IgG-Alexa Fluor 488. Scale bar: 10 μm. Error bars: mean ± SD (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001