Fig. 4

PTPN enhanced thrombolytic activity of tPA. a Thrombolytic activity of PBS, tPA, PM, TPN and PTPN (10 μg) at 1 h and 3 h incubation in vitro (n = 4). b Quantitative absorbance of lysed red blood cells after 3 h incubation at 538 nm (n = 4). c Representative photographs of femoral artery thrombus treated with normal saline, tPA, TPN and PTPN. d Images of H&E staining of femoral artery thrombus (n = 4). Scale bar = 100 μm. e Quantitative results of thrombus size in femoral artery. NS: normal saline. Data are presented as mean ± SEM. Statistical analysis was via one-way ANOVA with GraphPad Prism 8.0; ns: not significant; *p < 0.05; **p < 0.01; ***p < 0.001and ****p < 0.0001