Fig. 5
PTPN eliminated ROS and protected mitochondrial morphology. a Mitochondrial morphology (MitoTracker, red) and ROS level (DCFH-DA, green) of H2O2 damaged primary cardiomyocytes treated with PTPN (n = 3; scale bar = 20 μm). b TEM images of primary cardiomyocytes; red arrows indicate intact mitochondria. Scale bar = 2 μm in whole field and 1 μm in enlarged scale. c Quantitative results of ROS positive primary cardiomyocytes per field in image (a) (n = 4). d Average mitochondria length of primary cardiomyocytes in TEM (n = 20). e Average number of mitochondria of primary cardiomyocytes via TEM (n = 4). Data are presented as mean ± SEM. Statistical analysis was via one-way ANOVA with GraphPad Prism 8.0; ns: not significant; **p < 0.01; ***p < 0.001 and ****p < 0.0001