Fig. 4

Reversing MDR of nanoassemblies (A) Western blot of P-gp expression in A549/T cells after treated with PP, PP/NC-siRNA, PP/mdr1-siRNA, PP/HA, PP/NC-siRNA/HA, PP/mdr1-siRNA/HA and 25 K PEI/siRNA (B) Relative quantification of mdr1-gene expression by q-PCR analysis. C Cytotoxicities of PTX in A549 and A549/T cells. Cell viability treated with various concentrations of PTX and nanoassemblies. D A549 cells, E A549/T cells. (F) Western blot of LC3 and p62 expression in A549/T cells after treated with nanoassemblies for 48 h. G, H Quantitative presentation of Western blotting. T test compared to NC group. I CLSM images of GFP-LC3 positive dots in A549T/GFP-LC3 treated with nanoassemblies for 48 h. Scale bars: 20 μm. J Quantified result of GFP-LC3 positive puncta. K The acid phosphatase activity in A549/T cells by nanoassemblies for 48 h. The data are presented as means ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001