Fig. 5

Autophagy modulation of nanoassemblies (A) Western blot of LC3 and p62 expression after incubated with various concentrations of PP (0–50 μM), 10 μM CQ as positive control. B, C Quantitative presentation of Western blotting. D CLSM images of GFP-LC3 positive dots (green) and p62 immunofluorescence staining (red) in A549T/GFP-LC3 incubated with various concentrations of PP. Cell nucleus were stained by DAPI. Scale bars: 20 μm (E) Quantified result of GFP-LC3 positive puncta. F The quantitative fluorescence intensity of p62. G The acid phosphatase activity after treatment of PP. H Bio-TEM images of A549/T cells after incubated with PP (50 μM) and PP/siRNA/HA (50 μM) for 24 h. The arrows indicate the autophagosomes. The data are presented as means ± SD. *p < 0.05, **p < 0.01, and ***p < 0.001