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Fig.5 | Journal of Nanobiotechnology

Fig.5

From: Antenna-enhanced mid-infrared detection of extracellular vesicles derived from human cancer cell cultures

Fig.5

a 3D sketch of the small extracellular vesicles immunocapture on the functionalized nanoantenna array. b Ratio between reflectance spectra R(t), taken at time t after EVs exposure at t = 0, and R(0). The increasing blue color intensity represents the increasing t from 0 to 14 min with 1.3-min steps. The black line is a representative S-shaped fitting curve used to evaluate the SPR frequency shift. c Detailed view of the difference absorption signal in the region of the amide bands (1500–1700 cm−1), same color legend of panel b. d SPR frequency shift vs time obtained by the fitting procedure of the data in panel b in the 1700–2000 cm−1 range. The solid blue line represents the sigmoidal fitting curve. e Integral of the difference absorption signal in the amide band region vs t and the related sigmoidal fitting curve

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