Fig. 1

Recruitment and polarization of macrophages in the uterus of PROM model and normal control mice. A HE staining to analyze monocyte numbers in the same period in gestational uterus derived from normal control and PROM model mice. Representative monocytes are indicated with black arrows. B and C Anti-F4/80 antibody assessment and quantification of the number of macrophages in uteri. D and E Flow cytometry and counting of macrophages in amniotic fluid. F Representative immunofluorescence images of iNOS- or CD206-positive cells in the uteri. G Quantification of iNOS- and CD206-positive cells in the uteri. Scale bar = 100 μm. H mRNA levels of MMPs in F4/80-positive cells derived from normal control and PROM model mice. I, J and K ELISA detection of MMP-9, MMP-2, and MMP-13 expression in amniotic fluid; *p < 0.05; **p < 0.01; ***p < 0.001