Fig. 3

NF-κB pathway enhancement of transcription of B4galnt1, the host gene of cir2047, and miR-1931 in macrophages. A–C Expression of cir2047, miR-1931, and miR-760 measured by qPCR in F4/80-positive cells at different time points after polarization. D Immunofluorescence analysis of NF-κB (p65) and NF-κB1 (p50) in F4/80-positive cells after M1 polarization. E Schematic of each NF-κB1 (p50)-binding site (pBS) in the promoter regions of B4galnt1 and miR-1931. F and G Effect of NF-κB1 (p50) on B4galnt1 and miR-1931 transcription through binding to their promotors was evaluated by luciferase reporter assays. H and I EMSA of physical binding of NF-κB1 (p50) to the B4galnt1 and miR-1931 promotor regions; *p < 0.05; **p < 0.01; ns, not significant