Fig. 3
Peroxidase activity of CPCCM NPs. A, B UV–vis spectra of TMB (1 mM) with CPCCM at different pH values [H2O2] = 1 mM. C O2 concentration measurement by Dissolved Oxygen Meter in PBS (pH = 6.8) with CPCCM (50 μg/mL) and H2O2 (1 mM). D Absorbance of 50 μg/mL CPCCM NPs with the addition of 1.5–15 mM H2O2. E Michaelis–Menten kinetics of CPCCM NPs. F Lineweaver–Burk plotting of CPCCM NPs. G ESR analysis of 1O2 capture by TEMP (640 μΜ). H Singlet oxygen (1O2) generation ability is determined by SOSG (5 μΜ). I The CS-1 release from CPCCM NPs at pH 7.4 and pH 6.8. J The CS-1 release from CPCCM NPs under 660 nm laser irradiation (0.2 W/cm2). K Cell viability of MDA-MB-231 cells treated with different pH and CPCCM NPs (50 μg/mL) under 660 nm laser irradiation (0.2 W/cm2). Data were shown as mean ± SD, n = 3. ANOVA was used to assess statistical significance. ***p < 0.001