Fig. 4

The cellular uptake of Cap-GFP NPs and Cap-E2 NPs by BM-DCs. A Cytotoxicity of different proteins on BM-DCs after incubation for 24 h. Data was represented as cell viability (% of the control, Mean ± SD). B ST-GFP or Cap-GFP NPs was incubated with BM-DCs for 6 h, levels of internalization were analyzed by inverted fluorescence microscope. C levels of internalization were also assessed by flow cytometer and shown as mean fluorescence intensity (MFI). D BM-DCs were incubated with E2-ST or Cap-E2 NPs for 6 h, and then subject to immunofluorescence using CSFV E2 monoclonal antibody 3C12 and FITC-labeled donkey anti-mouse IgG. Finally, the nuclei (blue) and E2 (green) signal were recorded by laser confocal microscopy. *p < 0.05