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Fig. 5 | Journal of Nanobiotechnology

Fig. 5

From: A modular and self-adjuvanted multivalent vaccine platform based on porcine circovirus virus-like nanoparticles

Fig. 5

BM-DC costimulatory molecules and cytokines expression induced by Cap-SC based nanoparticles. A After incubation with E2-ST, Cap VLPs or Cap-E2 NPs for 6 h, BM-DCs were subject to immunofluorescence using FITC-labelled anti-mouse CD40 or CD86 antibodies, and assessed by flow cytometer and shown as mean fluorescence intensity (MFI). Total RNAs were isolated from E2-ST, Cap VLPs or Cap-E2 NPs treated BM-DCs, and mRNA levels of proinflammatory cytokines TNF-α (B), IL-12 (C) and IL-6 (D) were determined in qPCR, and the data were presented from triplicate experiments. ns, p > 0.05; *p < 0.05; **p < 0.01

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