Fig. 6

Influence of cytoskeletal structures on nuclear Ki67 activity and mechanotransduction. A Immunofluorescent images of Ki67 staining (green). Nuclei: blue; Actin: red. Scale bar: 50 μm. B Percentage of Ki67-positive cells in all checked cells. The data present the mean ± SD, n = 5, N.S. present no significance, *p < 0.05, **p < 0.01. C Immunofluorescent images of YAP staining (green). Nuclei: blue. White dotted circles present the outline of MG63 cells. Scale bar: 50 μm. D Percentage of YAP nuclear localization. The data present the mean ± SD, n = 5, N.S. present no significance, *p < 0.05, ***p < 0.001. E Illustration of micropattern-induced cytoskeleton nanomechanics to regulate gene transfection of MG63 cells