Fig. 6

Transcriptomic analysis and PDT-induced pyroptosis of primary tumor. A Volcano plot of DEGs in the PBS vs PDT. B KEGG analyses of DEGs in the PBS vs PDT. C Heatmap of the pyroptosis-related gene expression based on transcriptomic analysis data of primary tumor. TPM is an expression measure normalized to sequencing depth. Genes that did not significantly changed in each comparison are colored white. D PPI network analysis of pyroptosis-related genes. E–J Verification of the expression of Gsdmd (E), Caspase-1 (F), Nlrp3 (G), Asc (H), IL-18 (I) and IL-1β (J) at the mRNA level. K Protein expression of GSDMD-N, Cleaved-Caspase-1, NLRP3, and IL-1β in different groups. Data are expressed as means ± s.d. (n = 5). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001